The atomic structure of Carnation Mottle Virus capsid protein
نویسندگان
چکیده
منابع مشابه
Nucleotide sequence and genome organization of carnation mottle virus RNA.
The complete nucleotide sequence of carnation mottle genomic RNA (4003 nucleotides) is presented. The sequence was determined for cloned cDNA copies of viral RNA containing over 99% of the sequence and was completed by direct sequence analysis of RNA and cDNA transcripts. The sequence contains two long open reading frames which together can account for observed translation products. One transla...
متن کاملAn encapsidated, subgenomic messenger RNA encodes the coat protein of carnation mottle virus.
The translation strategy of carnation mottle virus (CarMV) in vitro has been generally assumed to involve internal initiation events on full-length, genomic RNA (4.3 kb). We suggest that this is, at least in part, incorrect. Encapsidated RNA, fractionated on denaturing sucrose gradients, or total RNA from CarMV-infected leaves, fractionated under non-denaturing conditions, was translated in an ...
متن کاملStructural properties of carnation mottle virus p7 movement protein and its RNA-binding domain.
Plant viral movement proteins (MPs) participate actively in the intra- and intercellular movement of RNA plant viruses to such an extent that MP dysfunction impairs viral infection. However, the molecular mechanism(s) of their interaction with cognate nucleic acids are not well understood, partly due to the lack of structural information. In this work, a protein dissection approach was used to ...
متن کاملPrimary structure of belladonna mottle virus coat protein.
The coat protein of belladonna mottle virus (a tymovirus) was cleaved by trypsin and chymotrypsin, and the peptides were separated by high performance liquid chromatography using a combination of gel permeation, reverse phase, and ion pair chromatography. The peptides were sequenced manually using the 4-N, N-dimethylaminoazobenzene-4'-isothiocyanate/phenyl isothiocyanate double-coupling method....
متن کاملgenome analyses of carnation mottle virus iranian isolate (fum2)
abstract during the winter and spring of 2007 and 2008, 450 samples (leaves of carnation plants) showing symptoms of yellowing, mottling, leaf malformation, and stunting were collected from greenhouses in mashhad and chenaran regions. the samples were tested by das-elisa for the presence of carmv. total rna was extracted by rnxtm (-plus) solution from positive infected samples confirmed in das-...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1994
ISSN: 0014-5793
DOI: 10.1016/0014-5793(94)80281-5